Coding

Part:BBa_K3748002:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


Alpha amylase from Bacillus subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 730
    Illegal SpeI site found at 22
    Illegal PstI site found at 86
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 730
    Illegal SpeI site found at 22
    Illegal PstI site found at 86
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 730
    Illegal BglII site found at 1202
    Illegal BglII site found at 1372
    Illegal BamHI site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 730
    Illegal SpeI site found at 22
    Illegal PstI site found at 86
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 730
    Illegal SpeI site found at 22
    Illegal PstI site found at 86
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codom optimization and Golden Gate Assembly compatible

Source

Uniprot

References

https://www.uniprot.org/uniprot/P00691

Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol., 4, 975–986.